/MitoFreSHtracer GSH Real-Time Assay Kit

MitoFreSHtracer GSH Real-Time Assay Kit


  • MitoFreSHtracerTM measures mitochondrial Glutathione (GSH)  in real-time.
  • Recommended for GSH measurement of Stem Cells
  • Quantifiable GSH levels through FACS or Confocal Microscopy
  •  1.0mg Tube of MitoFreSHtracerTM Powder
  • 650 well assays (96 well plate) or 100 FACS assays (6 well plate) / Tube


Product Type: Small Molecule
Name: MitoFreSHtracerTM
Molecular Weight: 1010.0
Molecular Formula C56H55Cl2N6O6P
Purity: ≥97% HPLC
Spectral Information
MitoFreSHtracer 520/580,
MitoFreSHtracer-GSH 430/510
Solubility: DMSO
Incubation Time:  60 minutes
Compatibility: All living cells
Shipping: Room Temperature
Storage: Room Temperature (up to 6 months)
DMSO Solution:  -20C (up to 6 months)
Appearance: Dark red solid
Platform: Operetta High-Content Imaging System
Confocal microscopy
Comments: MitoFreSHtracerTM is incorporated into the mitochondria inner membrane.  The unincorporated MitoFreSHtracerTM can be removed from cell culture media to produce a clearer backgound.

1.Real-Time Monitoring of Glutathione in Living Cells Reveals that High Glutathione Levels

 Are Required to Maintain Stem Cell Function.

Jeong EM, Yoon JH, Lim J, Shin JW, Cho AY, Heo J, Lee KB, Lee JH, Lee WJ, Kim HJ,
Son YH, Lee SJ, Cho SY, Shin DM, Choi K, Kim IG.

Stem Cell Reports. 2018 Jan 4