//FreSHtracer™
FreSHtracer™ 2018-01-29T10:58:24+00:00

For non-scientists, we recommend that you first take a look at our information on Reactive Oxygen Species (ROS) and Glutathione (GSH).

FreSHtracer™ Logo

(Fluorescence real-time thiol(SH) tracer)

  • Measures Glutathione in Real-Time
  • Works in All Cell Types
  • Simple and Fast Analysis
  • Requiring Only Small Amounts of Sample
FreSHtracer GSH Hela Cell
Real-time GSH measurement with FreSHtracer

Glutathione recovery after oxidant stress can be seen in this Real-Time Video using FreSHtracerTM

FreSHtracer™ is the first newly-designed fluorescent dye in the world to safely measure the level of Glutathione(GSH) in living cells. FreSHtracer™ is a small molecular probe that easily penetrates into cells and all cellular components. FreSHtracer™ works by binding to the Thiol (-SH) group of GSH.

The reaction between FreSHtracer™ and Glutathione(GSH) showing its reversibility.

When FreSHtracer™ is bound to GSH, it fluoresces at a peak of 510nm (F510), and when it unbinds from GSH, it continues to fluoresce at a peak of 580nm (F580). By measuring the fluorescent amount of F510 to F580, we can measure the amount of GSH throughout the cell. The binding and unbinding of FreSHtracer™ to GSH is completely reversible, so it will not consume the cell’s GSH by measuring it.

F510 and F580

FreSHtracer™ exhibits a concentration-dependent fluorescent intensity – the more GSH bound to the dye, the brighter it gets.

Ratiometric FreSHtracer

FreSHtracer™ is ratiometric! GSH is measured by the level of bound GSH to FreSHtracer™ (F510) and unbound FreSHtracer™ (F580).

FreSHtracer works in all cells

FreSHtracerTM is capable of measuring GSH in All Cell Types.

All cells have different levels of GSH.   With a FACS machine, sort cells into 3 categories using FreSHtracer™

Sort and select cells by their GSH levels (See our GSH-High Cell Therapy). A valuable quality control tool to monitor cell passaging and ensure GSH levels are maintained.  

GSH FACS Sorting
FACS sorting by glutathione levels
Purchase FreSHtracer Real-Time GSH Assay Kit

At Cell2in, we are currently developing other organelle-specific fluorescent FreSHtracer™ probes.

MitoFreSHtracer Logo
Measurement of Mitochondrial GSH
GolgiFreSHtracer Logo
Measurement of the Golgi Apparatus GSH
FreSHtracer-diAM Logo
Longer, Clearer Background, Measurement of Whole Cell GSH

FreSHtracer™’s Advantage Over Other Methods

  1. In High-performance liquid chromatography and Capillary electrophoresis, cells must be lysed(destruction of the cell membrane).
  2. Ellman’s Reagent, Monochlorobimane, and Monobromobimane irreversibly react with GSH making real-time measurement impossible.
  3. Grx1-roGFP is a large protein sensor that utilizes a virus to gain entry into cells but does not get absorbed by every cell and only measures levels of GSSG.
Find out how FreSHtracer is used in Stem Cell Therapy and Drug Screening